Quantification of toxic metals in chicken egg and chicken feed via SOM-artificial neural network.

Poultry products such as meat and eggs are rich sources of proteins, vitamins, and minerals. It is a good indicator of healthy food. Keeping in view, the present study is designed to evaluate the prevalence of toxic heavy metals (lead, nickel, cadmium, and chromium) in chicken eggs and feed. For this purpose, five samples of egg and feed were collected from five different commercial markets in Skardu City. Each sample was prepared using the wet digestion method and analyzed using a flame atomic absorption spectrophotometer. The results showed that lead, nickel, and chromium were present in varying amounts in the feed and egg, with nickel being the most concentrated metal, followed by lead and chromium in egg samples, while the feed samples showed the highest concentration of chromium followed by lead and nickel. However, concentrations of selected heavy metals except cadmium were all above the permissible limit of the World Health Organization. The self-organizing map-artificial neural network is employed for the identification of patterns of heavy metals in chicken feed and egg samples. The lower left neurons of the maps showed higher heavy metal concentrations found in samples taken from Bazar, whereas the rest of the samples showed varied concentrations. A comparison of feed and egg concentrations showed that nickel concentration was lower in feed samples than in egg samples. The lead concentration decreased in eggs except in the Krasmathang feed sample. Chromium concentration presented a negative correlation due to the extremely high concentration found in the Bazar feed sample.

Comparative genomics of light harvesting chlorophyll (LHC) gene family and impact of chlorophyll-A contents under drought stress in Helianthus annuus.

Drought is one of the main environmental stressors that can alter the water status of plants; negatively affect growth, assimilation, and photosynthesis; and eventually reduce crop yield. We explored the dependence of drought tolerance traits on chlorophyll-A content. Local sunflower cultivars (FH-01, FH-628, FH-633, FH-572, and FH-653) were grown in pots and subjected to drought by withholding water for 10, 15, or 20 d. One month after germination, the leaves of the treated and non-treated plants were collected and subjected to biochemical analyses. Under different water stress levels, the levels of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and proline increased, whereas those of chlorophyll-A decreased. Regression analysis clearly found that proline (-0.442), POD (-0.528), SOD (-0.532), and CAT (-0.814) have negative beta coefficient values. Phylogenetic analysis revealed that the LHC gene family is divided into six clades. Subcellular locations indicated that most LHC genes were located in the chloroplast; however, only few genes were present in the peroxisomes and endoplasmic reticulum. Our research found that Arabidopsis thaliana LHC genes were highly homologous to the LHC genes of Helianthus annuus. Furthermore, the LHC genes of both species are located in the chloroplasts; therefore, they play a role in photosynthesis and renewable energy production. This study opens a new horizon for discussing the role of chlorophyll-A in the drought-related traits of sunflowers.

Photodegradation of Rhodamine B using gallium hybrids as an efficient photocatalyst.

Keeping in view the toxicity of the Rhodamine B, the present study is designed to remediate the water loaded with toxic dyes using gallium oxide and gallium hybrids as photocatalyst. Precipitation coupled with sonochemical method is adopted for the synthesis of gallium oxide while the post grafting method is adopted for the synthesis of gallium hybrids with the indole and its derivatives. FTIR spectra showed the characteristic absorption bands of gallium oxide and gallium hybrids at 400-700 cm-1 and 1400-1600 cm-1. SEM and XRD showed the micro-sized rectangular rod-shaped gallium oxide with rhombohedral geometry. The average crystallite size of gallium hybrids was 26-32 nm calculated using the Debye Scherrer and Williamson-Hal models. The BET isotherm of gallium hybrids revealed the adsorption type-IV and hysteresis loop (H3) proposing multilayer and mesoporous structures with increase in surface area from 26 m2/g of gallium oxide to 31 m2/g of gallium-indole, 35 m2/g of gallium-methyl indole, and 37 m2/g of gallium-carboxylic indole. XPS showed the presence of gallium (3-14%), oxygen (28-32%), nitrogen (23-46%), and carbon (9-46%). The gallium oxide and gallium hybrids showed 47-72% optimum degradation of Rhodamine B under 2 h of illumination at pH 7 and 0.03 mg/L. The degradation rate followed a Langmuir-Hinshelwood model with R2 > 0.9.

Fabrication of polysulfone mixed matrix membrane for wastewater treatment.

Recent development in separation technologies has envisioned a green and sustainable future that encouraged energy preservation and waste minimization. The concept of a clean future emphasizes on retrieval and reutilization of valuable products from waste streams to improve the water quality. Membrane-based separations are currently explored as an auspicious substitution over traditional separation processes. The present study is designed to purify water using aluminum and gallium mixed matrix membranes from toxic metals (Lead and Mercury) and dyes (Rhodamine B, and Reactive Blue-4). Facile protocol i.e., immersion precipitation phase inversion method was used for the fabrication of mixed matrix membrane. The aluminium and gallium hybrids act as a filler and create heterogeneity and hydrophilicity within the membrane, affirming better water permeability and mechanical strength. The performance of fabricated mixed matrix membranes is evaluated using a lab-based dead-end membrane filtration unit. The result showed 30-71% rejection of Mercury, 24-65% rejection of Lead, 12-66% rejection of Reactive Blue-4, and 15-80% rejection of Rhodamine B.

Impact of transformational leadership intervention of head nurses among front-line soldiers (staff nurses) working in government hospital.

OBJECTIVE: To assess the impact of transformational leadership intervention among head nurses on staff nurses in improving job performance. Method: The quasi-experimental study was conducted from December 2020 to August 2021 at the public-sector Services Hospital, Lahore, Pakistan, and comprised head nurses and staff nurses enrolled through multistage cluster random sampling technique. Sixteen weeks of transformational leadership interventions were organised in morning and evening shifts. Pre- and post-intervention assessments were done. Data was analysed using SPSS 25. RESULTS: Of the 576 subjects, 480(83.3%) were staff nurses and 96(16.6%) were head nurses. Transformational leadership intervention caused a significant improvement in staff nurses' job performance owing to improvement in the behaviour of the head nurses behaviour (p<0.05). CONCLUSIONS: The study showed that staff nurses' performance significantly improved after transformational.

Sonochemical synthesis of aluminium and aluminium hybrids for remediation of toxic metals.

Spherical shaped nano-size aluminium oxide and its hybrids with indole and indole derivatives have been synthesized using sol-gel and post grafting methods coupled with sonication (Branson Digital SonifierS-250D; 20 kHz; 40%) for the remediation of toxic metals (lead and mercury). Different spectroscopic techniques (FTIR, SEM, BET, XRD, and XPS) have been applied to assess the properties of synthesized aluminium oxide and its hybrids. FTIR spectra showed the absorption bands of aluminium oxide (Al-O-Al) and aluminium hybrids (Al-O-C) at 800-400 cm-1 and 1650-1100 cm-1 region, respectively. SEM showed spherical shaped clusters of aluminium oxide which changed into the net-shape structure after the hybrid synthesis. It is worth noting that sonication energy increases the total surface area of aluminium oxide when it gets hybridized with indole and its derivatives from 82 m2/g to 167 m2/g; it also improved the product yield from 68% to 78%. Simultaneously, FTIR, SEM and BET analysis of non-sonicated aluminium oxide and its hybrids were also recorded for comparison. While XRD and XPS analysis were only conducted for sonicated aluminium oxide and its hybrids to manifest the structural and compositional properties. XRD patterns indexed as the cubic crystal system with an average 41 nm crystallite size of sonicated aluminium oxide which remains unaffected after hybrid synthesis. A survey scan under XPS confirmed the presence of all expected elements (aluminium, oxygen, carbon, nitrogen) and deconvolution of each recorded peak showed binding of element with its neighboring elements. The performance of aluminium oxide and its hybrids synthesize with and without sonication are also evaluated using a time-dependent batch adsorption protocol optimize for one hour. The maximum adsorption of lead (37%) and mercury (40%) are found onto sonicated aluminium oxide. The sonicated aluminium hybrids showed 43-63% of lead and 55-67% of mercury at pH 7. The fitness of experimental data using adsorption kinetics and isotherms revealed that adsorption follows Pseudo-second-order kinetic, Langmuir, and Freundlich isotherms.

Determining the association of high-commitment human resource practices with nurses' compassionate care behaviour: A cross-sectional investigation.

AIM: The aim of this study was to determine the association of high-commitment human resource practices (HCHRPs) with nurses' compassionate care behaviour. BACKGROUND: The extant research highlights the key role of compassionate care behaviour in achieving quality health services. However, scant attention has been paid to identifying the factors that augment compassionate care behaviour among health care professionals. METHODS: This study took place from September 2018 to March 2019, collecting data from 340 nurses of either gender aged 24-60 years with at least 1 year experience. The study follows a cross-sectional research design by collecting data through a personally administered survey. RESULTS: A statistically significant association was found between the four dimensions of HCHRPs and organisational commitment. Moreover, organisational commitment positively related to compassionate care behaviour and mediated the relationship between HCHRPs and compassionate care behaviour. CONCLUSION: The findings reveal that four dimensions of HCHRPs positively influence nurses' compassionate care behaviour via the indirect path of organisational commitment. IMPLICATIONS FOR NURSING MANAGEMENT: The study marks the first attempt to delineate the role of HCHRPs in enhancing the compassionate care behaviour. The hospital administration and human resource managers should use HCHRPs as strategic imperative to attract competent employees and enhance compassionate care behaviour.

Ultrasound-assisted synthesis of gallium hybrids for environmental remediation application.

Micron-sized, rhombohedral shaped gallium hybrids with different indole derivatives (indole, 2-methyl-indole, indole-2-carboxylic acid) were successfully synthesized with precipitation method coupled with ultrasound followed by the post-grafting method. The as-synthesized hybrid materials were characterized using FTIR, SEM, XPS, XRD, and BET techniques. FTIR spectra showed characteristic absorption bands of gallium oxide and gallium hybrids at 400-700 cm-1 and 1400-1600 cm-1. SEM, XRD, and BET showed that ultrasound-assisted gallium micro-particles are porous, crystalline possessing high surface to volume ratio as compared to that synthesized in the absence of ultrasound. Survey scan of XPS revealed the presence of gallium, oxygen, nitrogen, and carbon. The as-synthesized gallium hybrids were applied as a potential photocatalyst towards Reactive Blue 4 (model pollutant) using batch adsorption experiment under visible light. It showed maximum 30-65% degradation within two hours and followed a pseudo-first-order kinetic model with R2 > 0.9.

Congenital bleeding disorders in Karachi, Pakistan.

OBJECTIVE: To determine the frequency of inherited bleeding disorders, its complications, and treatment modalities available for its treatment. DESIGN: Cross-sectional study. PATIENTS AND METHODS: Patients with a history of bleeding tendency were tested for confirmation of the diagnosis. History and clinical findings were recorded. Laboratory analysis included prothrombin time (PT), activated partial thromboplastin time (APTT), bleeding time (BT), and fibrinogen assay. Patients with prolonged APTT were tested for factors VIII (FVIII) and IX (FIX). If FVIII was low, von Willebrand factor: antigen (vWF:Ag) and von Willebrand factor:ristocetin cofactor (vWF:RCo) were performed. When PT and APTT both were prolonged, FV, FX, and FII were tested. Platelet aggregation studies were done when there was isolated prolonged BT. Urea clot solubility test was done when all coagulation tests were normal. All patients with hemophilia A and B were evaluated for inhibitors. RESULTS: Of the 376 patients, inherited bleeding disorder was diagnosed in 318 (85%) cases. Median age of patients was 16.4 years. Hemophilia A was the commonest inherited bleeding disorder that was observed in 140 (37.2%) followed by vWD 68 (18.0%), platelet function disorders 48 (12.8%), and hemophilia B in 33 (8.8%) cases. We also found rare congenital factor deficiencies in 13 (3.4%), low VWF in 11 (3.0%) participants and 5 (1.3%) in female hemophilia carriers. Hemarthrosis was the most frequent symptom in hemophilia A and B (79.7%) involving knee joint. Inhibitor was detected in 21 (15%) cases. Fresh frozen plasma/cryoprecipitate were the most common modality of treatment. In 58 patients, no abnormality was detected in coagulation profile. CONCLUSION: Hemophilia A and vWD are the most common congenital bleeding disorders in this study. Hemarthrosis involving knee joint was the most common complication. Inhibitor was detected in a significant number of patients. Plasma is still the most common modality of treatment.